Cigarette Smoke Exposure Leads to Organic and Mineral Bone Component Changes: The Importance of Rho Kinase Function in These Events

Cigarette Smoke Exposure Leads to Organic and Mineral Bone Component Changes: The Importance of Rho Kinase Function in These Events

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Aberrant Rho-associated kinase function could be associated with increased bone fragility.Since cigarette smoke (CS) exposure promotes 2 Channel LOC the increase in bone fragility due to changes in bone tissue components, this study aimed to investigate how CS exposure could modulate the Rho kinase-associated bone structural changes.Mice were assigned to four groups: control; smoke; control with Rho kinase inhibitor administration; and smoke with a Rho kinase inhibitor.Bone samples were obtained to assess bone histomorphometry analysis, type I collagen composition, and MEPE expression in trabeculae.

We observed that CS exposure induced decreased trabecular and osteoid thickness.A concomitant increase in the osteoclastic and erosion surfaces and a decrease in the mineralization surface were observed.Additionally, CS exposure decreased the type I collagen and MEPE expression.Rho kinase inhibitor administration recovered the bone mineralization and the collagen type I deposition.

Conclusions: CS exposure increases Rho kinase activity Bases in bone cells, leading to structural changes.The administration of a Rho GTPases inhibitor partially reverses these effects, likely due to the recovery in osteoblast activity.